Fecal metagenome of pigs weaned at different ages - SRA

SRX10721841: Fecal metagenome of pigs weaned at different ages
1 ILLUMINA (Illumina NovaSeq 6000) run: 16.3M spots, 8.2G bases, 2.5Gb downloads

Design: Metagenomic libraries were prepared using 700 ng of DNA and the TruSeq DNA PCR-Free Library Prep Kit (Illumina Inc.) following the manufacturers recommended protocol. Briefly, DNA was fragmented to an average length of 400 bp with a Covaris LE220 instrument, end-repaired, A-tailed, and indexed with TruSeq Illumina adapters. Libraries were then validated for size and the abon a Fragment Analyzer system with a High Sensitivity NGS Fragment Kit (Agilent Technologies, Mississauga, ON, Canada) to check for size and quantified by qPCR using the Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems, Wilmington, MA, USA). Equimolar quantities of each library were then pooled and sequenced on the Illumina Novaseq 6000 instrument with a SP flowcell (2 x 250 bp) following manufacturers instructions.

Submitted by: Agriculture and Agri-Food Canada

Study: Weaning Piglet Gut Microbiome

PRJNA629856 • SRP262165 • All experiments • All runs

show Abstracthide Abstract

The effect of weaning age on the piglet gut microbiome was evaluated by collecting fecal swabs from piglets and sows. Piglets were weaned at 14 days, 21 days or 28 days and fecal swabs were collected from day four until the pigs reached market weight. Fecal swabs from the sows were taken from day zero and at each weaning date.

Sample:

SAMN17899690 • SRS8812239 • All experiments • All runs

Organism: pig gut metagenome

Library:

Name: H1P409W14FD070

Instrument: Illumina NovaSeq 6000

Strategy: WGS

Source: METAGENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 16.3M spots, 8.2G bases, 2.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR14369134	16,298,969	8.2G	2.5Gb	2021-05-06

ID:: 14253632

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